Imunohistochemical Localization of α-Amylase in Cotyledons of Vigna mungo Seedlings
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چکیده
منابع مشابه
Imunohistochemical Localization of alpha-Amylase in Cotyledons of Vigna mungo Seedlings.
We studied the localization of alpha-amylase with indirect fluorescence microscopy in transversely sectioned cotyledons of Vigna mungo seedlings. Tissue sections were fixed in periodate-lysine-paraformaldehyde and treated with anti-alpha-amylase immunoglobulin G followed by fluorescein isothiocyanate labeled goat anti-rabbit immunoglobulin G. alpha-Amylase appeared in the cells farthest from va...
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Pe]ypheno] oxidase (monophenol dLhydrexyphenylalanine' oxygen ox]doTeductase/ EC 1 14.1S t; PPO) hus been found in most higher plunts. although its ro]e has not yet becn clearly cstab]]shed. When the extracts frem var]ous organs of' mung bean seedlings were unalyzecl by :soelectric f'ocustng, several bancls of PPO ttet]vity were dctectcd. Among these isozyrnes, the one with pl S,4 showed the hi...
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Alpha-amylase is expressed at high levels in cotyledons of germinated seeds of Vigna mungo. The mRNA for alpha-amylase appeared in cotyledons of the seeds at 1 d after imbibition started (DAI). Two TGACGT motifs at -445 and at -125 in the promoter region of the gene interacted with nuclear proteins from cotyledons of dry seeds and the activities were detected until 3 DAI. A transient assay with...
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The effect of seaweed liquid fertilizer (SLF) of Sargassum myriocystum on germination, growth and biochemical constituents of Vigna mungo was studied. Different concentrations (5%, 10%, 25%, 50% and 75%) of SLF were prepared using distilled water. A total of 100 seeds were soaked for each SLF concentration for a 24-h period and were later placed in various Petri plates and watered regularly. Wa...
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ژورنال
عنوان ژورنال: Plant Physiology
سال: 1985
ISSN: 0032-0889,1532-2548
DOI: 10.1104/pp.79.4.935